Journal: Communications Biology

Article Title: Crosstalk of cell death pathways unveils an autophagy-related gene AOC3 as a critical prognostic marker in colorectal cancer

doi: 10.1038/s42003-024-05980-6

Figure Lengend Snippet: a Univariate Cox analysis of CDN4 feature genes with p < 0.05 and HR > 1 in TCGA and GEO prognosis cohorts. b Mean C-index of the candidate genes. c – f Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 group and low AOC3 group in TCGA and three validation datasets. g Representative immunohistochemistry (IHC) staining images of CRC tissue microarrays in low AOC3 tumor expression and high AOC3 tumor expression tissues. h Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 H-scores group and low AOC3 H-scores group in IHC arrays. i The molecules significantly associated with AOC3 were enriched in regulation of autophagy pathway. j Correlation analysis of AOC3 with regulation of autophagy pathways score. k Differential expression of autophagy genes in different AOC3 expressions. ns fdr>0.05, *fdr < 0.05, **fdr < 0.01, ***fdr < 0.001, ****fdr < 0.0001.

Article Snippet: Immunohistochemistry (IHC) was performed using AOC3 (1:600; Cat no. 66834-1-Ig, Proteintech, Wuhan, China).

Techniques: Biomarker Discovery, Immunohistochemistry, Expressing, Quantitative Proteomics